h3k4me1 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc h3k4me1

596 interacts with LSD1 in EC Cells. (A). The molecular structure of 596. (B). Western blot detection of <t>H3K4me1,</t> H3K4me2, and H3K4me3 after 596(0,4,8,12,16 μM) treatment( n = 3). (C-D). After treating ISK and HEC1A cells with DMSO and 596 (12 μM) for 2 h, the thermal stability of LSD1 was detected by CETSA method. The quantitation (right) of western blot results. (E). In ISK and HEC1A cells, detect the proliferation of compound 596 after 48–96 h of different concentrations by CCK8. (F-G). GraphPad Prism 9 was used to calculate IC50 of 596 in ISK and HEC1A cells. (H). ISK cells and HEC1A cells were treated with different concentrations of 596 respectively, and the proliferation of the cells was detected by colony formation assay.

H3k4me1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 257 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h3k4me1/Mono-Methyl-Histone+H3+(Lys4)+XP+Rabbit+mAb/pmc12914821-46-43-45
Average 96 stars, based on 257 article reviews

h3k4me1 - by Bioz Stars, 2026-10

96/100 stars

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1) Product Images from "The novel tertiary amine LSD1 inhibitor 596 inhibits endometrial cancer through the mTOR signal transduction pathway"

Article Title: The novel tertiary amine LSD1 inhibitor 596 inhibits endometrial cancer through the mTOR signal transduction pathway

Journal: Translational Oncology

doi: 10.1016/j.tranon.2026.102688

596 interacts with LSD1 in EC Cells. (A). The molecular structure of 596. (B). Western blot detection of H3K4me1, H3K4me2, and H3K4me3 after 596(0,4,8,12,16 μM) treatment( n = 3). (C-D). After treating ISK and HEC1A cells with DMSO and 596 (12 μM) for 2 h, the thermal stability of LSD1 was detected by CETSA method. The quantitation (right) of western blot results. (E). In ISK and HEC1A cells, detect the proliferation of compound 596 after 48–96 h of different concentrations by CCK8. (F-G). GraphPad Prism 9 was used to calculate IC50 of 596 in ISK and HEC1A cells. (H). ISK cells and HEC1A cells were treated with different concentrations of 596 respectively, and the proliferation of the cells was detected by colony formation assay.

Figure Legend Snippet: 596 interacts with LSD1 in EC Cells. (A). The molecular structure of 596. (B). Western blot detection of H3K4me1, H3K4me2, and H3K4me3 after 596(0,4,8,12,16 μM) treatment( n = 3). (C-D). After treating ISK and HEC1A cells with DMSO and 596 (12 μM) for 2 h, the thermal stability of LSD1 was detected by CETSA method. The quantitation (right) of western blot results. (E). In ISK and HEC1A cells, detect the proliferation of compound 596 after 48–96 h of different concentrations by CCK8. (F-G). GraphPad Prism 9 was used to calculate IC50 of 596 in ISK and HEC1A cells. (H). ISK cells and HEC1A cells were treated with different concentrations of 596 respectively, and the proliferation of the cells was detected by colony formation assay.

Techniques Used: Western Blot, Quantitation Assay, Colony Assay

Incubation:

Article Title: The pan-cancer mutational landscape of MLL3 and its impact on prognosis and immunochemotherapy.
Article Snippet: .. The ConA bead-cell complexes were then incubated overnight with specific primary antibodies against histone modifications H3K27ac (Abcam, ab4729) and H3K4me1 (Cell Signaling, 5326S). ..

Article Title: Multidimensional mapping of stimulation-responsive regulatory elements and candidate causal variants in T cell activation
Article Snippet: Nuclear lysates were sonicated to generate DNA fragments ranging from 200 to 500 bp using a Biorupter system (Diagenode, B01060001) at high energy, with a working time of 30 s and a resting time of 30 s, for a total of 30 cycles, until the solutions became clear. .. After removing insoluble debris, lysates were incubated with specific antibodies against Rabbit IgG (1:100, Cell Signaling Technology, 7074), CTCF (1:100, Abcam, ab128873), ZNF384 (1:100, Novus Biologicals, NBP3-30592), H3K27me3 (1:100, Cell Signaling Technology, 9733), Rpb1 NTD (1:100, Cell Signaling Technology, 14958), H3K4me1 (1:100, Cell Signaling Technology, 5326), H3K4me3 (1:100, Cell Signaling Technology, 9751), or H3K27ac (1:100, Abcam, ab4729). .. Antibody-bound complexes were purified using protein A–agarose beads (Millipore, 16-157).

Immunoprecipitation:

Article Title: High-throughput functional characterization of enhancers in totipotent-like cells
Article Snippet: .. One microgram of histone H3K27ac (39133, Active Motif), H3K4me1 (5326S, Cell Signaling Technology) or H3K4me3 (9751S, Cell Signaling Technology) antibody was used for each immunoprecipitation reaction. .. The sequencing libraries were prepared using the NEBNext ® UltraTM II DNA Library Prep Kit for Illumina ® (E7645S, NEB) and the NEBNext ® Multiplex Oligos for Illumina ® (Index Primers Set 1) (E7335S, NEB) following the manufacturer’s instructions.

Article Title: Time-resolved multiomics profiling reveals chromatin O-GlcNAc modification promotes senescence-associated transcriptional program.
Article Snippet: .. After pre-clearing with vehicle-coated magnetic beads, chromatin complexes were immunoprecipitated with either anti-Flag magnetic beads, anti-HA magnetic beads, or antibodies against histone modifications, including H3K27me3 (CST, #9733, 1:50), H3K27ac (CST, #8173, 1:50), H3K4me1 (CST, #5326, 1:50), or H3K4me3 (CST, #9751, 1:50). ..

Article Title: Time-resolved multiomics profiling reveals chromatin O-GlcNAc modification promotes senescence-associated transcriptional program
Article Snippet: .. After pre-clearing with vehicle-coated magnetic beads, chromatin complexes were immunoprecipitated with either anti-Flag magnetic beads, anti-HA magnetic beads, or antibodies against histone modifications, including H3K27me3 (CST, #9733, 1:50), H3K27ac (CST, #8173, 1:50), H3K4me1 (CST, #5326, 1:50), or H3K4me3 (CST, #9751, 1:50). ..

Magnetic Beads:

Cell Culture:

Article Title: The novel tertiary amine LSD1 inhibitor 596 inhibits endometrial cancer through the mTOR signal transduction pathway.
Article Snippet: .. The EC cells were cultured in DMEM medium(Viva cell, China) containing 10% fetal bovine serum (Viva cell, China) at 37◦C and 5% CO2.The main purchasing information was listed below:LSD1 (1:10,000, ab129195, Abcam, UK), H3(1:20,000, No.4499T,Cell Signaling Technology, CST, Boston, MA, USA), H3K4me1(1:2000, No.5326T, CST), H3K4me2(1:2000, No.9725S, CST), H3K4me3(1:2000, No. 9751T, CST), p62/SQSTM1(1:2000, No.5114T, CST), LC3B(1:2000, Cat# AF4650, Affinity Biosciences), Beclin-1(1:2000, Cat# AF5128, Affinity Biosciences), AKT(1:5000, YM8463, Immunoway, USK), P-AKT (Ser473)(1:2000, YM8304, Immunoway, USK), mTOR(1:5000,YM8208, Immunoway, USK), P-mTOR(Ser2448)(1:5000, YM8326, Immunoway, USK), S6(1:2000, YT4139, Immunoway, USK), P-S6(1:2000, No.F0198, Selleck.cn), p70S6K(1:2000, YT3559, Immunoway, USK), P-p70S6K (1:1000, No.F2907, Immunoway, USK), GAPDH(1:5000, Proteintech). .. Rapamycin, 3-MA was purchased from MCE and CC9001 was purchased from KKL Med Inc.

Article Title: The novel tertiary amine LSD1 inhibitor 596 inhibits endometrial cancer through the mTOR signal transduction pathway
Article Snippet: .. The EC cells were cultured in DMEM medium(Viva cell, China) containing 10% fetal bovine serum (Viva cell, China) at 37°C and 5% CO 2 .The main purchasing information was listed below:LSD1 (1:10,000, ab129195, Abcam, UK), H3(1:20,000, No.4499T,Cell Signaling Technology, CST, Boston, MA, USA), H3K4me1(1:2000, No.5326T, CST), H3K4me2(1:2000, No.9725S, CST), H3K4me3(1:2000, No. 9751T, CST), p62/SQSTM1(1:2000, No.5114T, CST), LC3B(1:2000, Cat# AF4650, Affinity Biosciences), Beclin-1(1:2000, Cat# AF5128, Affinity Biosciences), AKT(1:5000, YM8463, Immunoway, USK), P-AKT(Ser473)(1:2000, YM8304, Immunoway, USK), mTOR(1:5000,YM8208, Immunoway, USK), P-mTOR(Ser2448)(1:5000, YM8326, Immunoway, USK), S6(1:2000, YT4139, Immunoway, USK), P-S6(1:2000, No.F0198, Selleck.cn), p70S6K(1:2000, YT3559, Immunoway, USK), P-p70S6K(1:1000, No.F2907, Immunoway, USK), GAPDH(1:5000, Proteintech). .. Rapamycin, 3-MA was purchased from MCE and CC9001 was purchased from KKL Med Inc.

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